Emergency services responded to the incident, but the 83-year-old was pronounced dead at the scene.
The accident happened on the A9 between Inveralmond and Broxden Roundabout just before 2.30am on Sunday.
A Police Scotland spokesman said: “At about 2.25am we responded to reports of a female pedestrian being struck by a vehicle on the A9.
Police have declined to give details of the incident and could not confirm if this was a hit and run.
Police have urged any witnesses to the incident to contact non-emergency 101, quoting incident number 0672 of November 4.